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How many cells? Nicholas Ostrout, Ph.D. |
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Friday, 25 July 2008 |
This is a question I hear often; "How many cells must I analyze for it to be statistically significant?" Although I haven't ever been quite sure of the answer to that question, I now have something concrete to provide thanks to a recent trip to Auburn and a posting I noticed on the wall. I'm sure others have wondered or heard this asked before, so I thought this might be helpful to share with everyone. I have actually made copies of it and also a pdf version to distribute if people would like it. You can also get it here . Thanks to Allison Church Bird at Auburn who got it from the Bowdoin Course. There is a reference at the bottom to 'Wallce and Muirhead', so we should probably send a thanks their way too! I'm sure there are other ways people determine how many cells they must collect for their results to be statistically significant, so if you have a better way or use something different, write about it in the forum. It would be great for us to start a discussion about it and pass the knowledge around. Cheers.
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Progress on the Analytical Cytometry Standard (ACS) by John Quinn Ph.D |
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Thursday, 24 July 2008 |
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MIFlowCyt, the minimum information required for reporting a flow cytometric experiment, was accepted in March of this year by ISAC as the standard detailing what information the Analytical Cytometry Standard (ACS) should contain, but specifies nothing regarding the format of the newest data file standard (Unfamiliar with the ACS? View my previous post here ). The discussion within our community has now turned to what that format will be. At the ISAC meeting in Budapest, the Data Standards Task Force (DSTF) hosted several discussions and assembled the attending members for a meeting in an attempt to gauge the prevailing opinions on what the format needs to be for ACS. The conclusion was that the ACS file itself should be a container file, meaning that it stores a number of other data containing files that can be of a variety of formats. The task force agreed that this was the best way to capture a large amount of metadata while recording the actual measurements in a format that could lack most header information and thus be read by a wide array of non-proprietary software.
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Rare Mutant Cells Glimpsed: Imaging System May Help Understand Origins Of Cancer |
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Wednesday, 23 July 2008 |
MIT biological engineers have developed a new imaging
system that allows them to see cells that have undergone a specific
mutation. The work, which could help scientists understand how precancerous
mutations arise, marks the first time researchers have been able to
pinpoint the number and location of mutant cells--cells with a
particular mutation--in intact tissue. In this case, the researchers
worked with mouse pancreatic cells.
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NIST trumps the clumps: Making biologic drugs safer |
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Wednesday, 23 July 2008 |
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Scientists at the National Institute of Standards and Technology
(NIST) have developed a technique to measure the formation of clumps of
proteins in protein-based pharmaceuticals. This first systematic study*
clarifies the conditions under which scientists can be assured that
their instruments are faithfully measuring the formation of protein
aggregates, a major concern because of its impact on quality control
and safety in biologic drug manufacturing.
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