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Register Standards Bioinformatics Standards Flow Cytometry Standard (FCS) 3.0 Proposal Flow Cytometry Standard (FCS) 3.0 MIFlowCyt 1.0 MIFlowCyt 1.0 Summary Analytical Cytometry Standard NetCDF Analytical Cytometry Standard Gating-ML Proposal Analytical Cytometry Standard Gating-ML (full) Cytometry Standards Continuum Protocols Manufacturer Protocols Invitrogen BD Biosciences Biocompare - Flow Cytometry Web Available Cell Cycle Analysis BrDU and PI Staining Yeast Cell Cycle Fixation and DNA CFDA-SE for cell tracking and cell cycle analysis Collecting Mouse Tissue Samples FACS Staining Protocols Standard FACS Surface Staining One and Two Color Methods Surface and Intracellular Whole Blood Intracellular (by IntraCyte) Intracellular Cytokine Human Antibody Titiration MCB Staining Mario\'s Conjugation Hemoglobin ELISA Staining Protocols Intracellular Antigen Propidium Idodide Staining of Dead Cells Minimizing Background Staining Monoclonal Antibody Staining Staining Procedure for Whole Blood 7-AAD Staining of Dead Cells Freezing and Thawing PBMC MACS Depletion Protocol Preparation of 2% Formaldehyde Hawley&Hawley FC Protocols 2nd Ed. Multiparameter Flow Cytometry of Bacteria Multiparameter Data Acquisition and Analysis of Leukocytes Flow Cytometric Analysis of Kinase Signaling Cascades Cytokine Flow Cytometry Use of Cell-Tracking Dyes in Frequencies of Antigen Specific T-Cells Lymphocyte-Mediated Cytotoxicity Measurement Simultaneous- Cell Surface IF, Viability and DNA Simultaneous- Dual-Color IF and DNA/RNA Content Simutaneous- Three-Color IF and DNA Content Simultaneous- Apoptosis and Necrosis GFP Expression and DNA Content in Permeabilized Cells GFP Expression and DNA Content in Unfixed Cells Intracellular Ca2+ and Cell Curface Antigen Expression Parafomaldehyde Preparation Preparation of PBMC from Buffy Coats Antibody Labeling Fluorescein Isothiocyanate (FITC) Long Armed Biotin Texas Red Fluorescein Labeling Phycobiliproteins Hapten-anti-hapten labeling Immunolabeling of stable cell nuclei by flow cytometry Fluorochrome conjugation by amine modification Tietze Analysis Immunofluorescence Direct Direct IF Direct IF Basic Direct (by abcam) Indirect Indirect IF Basic Indirect (by abcam) DNA Analysis of Human Cancer Cell Lines NTS Buffer/DAPI Stain Content Analysis Using Hoechst 33342 in Unfixed Cells Blocking FC-Receptor Binding Detection of calcium flux with indo-1 Cell Death/Apoptosis Cell cycle and apoptosis analysis using DNA binding dyes Cell death analysis using PhiPhiLux caspase 3 substrate and annexin V Detection of Apoptosis by 7-AAD Staining Current Protocols in Cytometry (Interscience Wiley) Educational Online Texts Practical Flow Cytometry 4th Ed., Shapiro (2005) Flow Cytometry Protocols 2nd Ed., Hawley & Hawley (2004) Cytometry 3rd Ed. Vol. 63, Darzynkiewicz Flow Cytometry Protocols, Jaroszeski and Heller (1998) Flow Cytometry for Biotechnology, Sklar (2005) Flow Cytometry with Plant Cells. Dolezel, Greilhuber and Suda (2007) Flow Cytometry: A Practical Approach, Ormerod (2003) Cytometric Analysis of Cell Phenotype and Function, McCarthy and Macey, (2003) Flow Cytometry: Principles and Applications, Macey (2007) Flow Cytometry in Hematopahtology, Nguyen, Diamond and Braylan, (2003) Introduction to Flow Cytometry Flow Cytometry Data Analysis Techniques in Cell Cycle Analysis Fluorochrome Tables Salk Institute Table Invitrogen Table Sciencegateway Table BD Biosciences Viewer Cytometry Tutorials and Presentations Video Tutorials Compensation Pt. 1 (Marty Bigos) Compensation Pt. 2 (Mario Roderer) Compensation Pt. 3 (Nicole Baumgarth) Cytometry Overviews by Invitrogen by BD Biosciences by J. 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Papaya extract thwarts growth of cancer cells in lab tests Papaya extract seems to have a toxic effect on cancer cells in culture, suggesting a potential treatment. Scientists documented for the first time that papaya... Sonic hedgehog gene found in an unexpected place during limb development Sonic hedgehog is at work in mice limb buds in what is known as the ectoderm, the cell layer that gives rise to skin, researchers... Hidden habits and movements of insect pests revealed by DNA barcoding University of Minnesota researcher George Weiblen and colleagues have found a faster way to study the spread and diet of insect pests. Using a technique... New study questions benefits of elective removal of ovaries during hysterectomy Removal of the ovaries (bilateral oophorectomy) while performing a hysterectomy is common practice to prevent the subsequent development of ovarian cancer. This prophylactic procedure is... Sources of pollution in waterways A new study reviews the possible sources of Polycyclic aromatic hydrocarbons (PAHs) pollution to the New York/New Jersey Harbor. Dome away from home After more than three decades of service to researchers and staff stationed at the bottom of the world, the dome at the Amundsen-Scott South Pole... Cotton is the fabric of your lights...your iPod...your MP3 player...your cell phone Consider this T-shirt: It can monitor your heart rate and breathing, analyze your sweat and even cool you off on a hot summer's day. Or... Tropical cyclone formation likely near Madagascar Forecasters are watching a low pressure area located off the east coast of Madagascar that appears ripe for development in the Southern Indian Ocean. If... After a fight with a partner, brain activity predicts emotional resiliency Neural activity in the lateral prefrontal cortex can predict whether an individual will still be upset on the day after a conflict with his or... Patients with Irritable Bowel Syndrome not more likely to develop polyps, colon cancer Patients with irritable bowel syndrome are at no greater risk of having polyps, colon cancer or inflammatory bowel diseases than healthy people undergoing colonoscopies. 'The Rosenfeld' named after California's godfather of energy efficiency A group of scientists propose today in a refereed article in Environmental Research Letters to define the Rosenfeld as a unit for electricity savings, after... Critical recommendations unveiled: Deep-vein thrombosis and pulmonary embolism Three critical recommendations from a national workshop have been released to address deep-vein thrombosis and pulmonary embolism, a growing public health problem estimated to affect... From the desk lamp to the desktop? In the future, getting a broadband connection might be as simple as flipping on a light switch. In fact, according to a group of researchers... Study finds cancer mortality has declined since initiation of 'war on cancer' A new American Cancer Society study finds progress in reducing cancer death rates is evident whether measured against baseline rates in 1970 or in 1990.... Grandfathered drug for high potassium has no proven benefit For more than half a century, products containing ion exchange resins have been used in patients with dangerously high levels of potassium. However, there is... Male batterers consistently overestimate rates of violence toward partners Men who engaged in domestic violence consistently overestimated how common such behavior is by two or three times, and the more they overestimated it... Divine intervention? New research looks at beliefs about God's influence in everyday life Most Americans believe God is concerned with their personal well-being and is directly involved in their personal affairs, according to new research out of the... Model may offer better understanding of embryonic development A mathematical model developed at Purdue University can predict complex signaling patterns that could help scientists determine how stem cells in an embryo later become... Study: Federal funds support health depts., but leadership is key The surge of funds for bioterrorism preparedness over the past decade does not appear to be improving local public health resources in general, according to... K-State professor finds link between low oxygen levels in body and cancer-aiding protein Dolores Takemoto, a K-State professor of biochemistry who was researching protein kinase C gamma in the lens of the human eye, found her work taking... Chemical competition: Research identifies new mechanism regulating embryonic development A Princeton University-led research team has discovered that protein competition over an important enzyme provides a mechanism to integrate different signals that direct early embryonic... ARS study provides a better understanding of how mosquitoes find a host The potentially deadly yellow-fever-transmitting Aedes aegypti mosquito detects the specific chemical structure of a compound called octenol as one way to find a mammalian host... Study finds that low-income women living in small cities have higher chance of obesity A recent Kansas State University study found that the availability of supermarkets -- rather than the lack of them -- increased the risk of obesity... NJIT prof names baseball winners With pitchers and catchers having recently reported to spring training, once again Bruce Bukiet, an associate professor at NJIT, has applied mathematical analysis to compute... Designer nano luggage to carry drugs to diseased cells UK scientists have succeeded in growing empty particles derived from a plant virus and have made them carry useful chemicals. The external surface of these... Researcher presents risk-free treatment for low female sexual desire Researchers are currently testing a new drug, flibanserin, which was developed as an antidepressant and affects neurotransmitters in the brain, to treat women with low... Intentional variation increases result validity in mouse testing For decades, the traditional practice in animal testing has been standardization, but a study involving Purdue University has shown that adding as few as two... Smelling the scenery in stereo Desert ants are well-known for their remarkable orientation: they use a compass along with a step counter and visible landmarks to locate their nest. After... The alpine marmot spreads into the Catalan Pyrenees Researchers from the Centre for Ecological Research and Forestry Applications and the Autonomous University of Barcelona have demonstrated, using a map of the potential distribution,... Plants discover the benefits of good neighbors in strategy against herbivores Scandinavian Scientists have discovered that a species of tree defends itself from herbivore attack by using chemicals emitted by neighboring plants. The study, published today... Female teachers ask for work/family reconciliation to be able to access management posts A research team made up of various Andalusian universities and coordinated by the University of Huelva has concluded that the main barrier that these professionals... Earthquake in Chile -- a complicated fracture The extremely strong earthquake in Chile on 27 February this year was a complicated rupture process. Scientists ofthe GFZ German Research Centre for Geosciences analyzed... Massage eases anxiety, but no better than simple relaxation does A randomized trial shows three months after 10 massages, Group Health patients' anxiety symptoms were halved -- an improvement like that previously reported with psychotherapy,... Laptop revolution: New class design saves schools money, space Universities around the country are struggling with shrinking budgets, even as they need to cater to the needs of an increasing number of students. New... NIAD media availability: Vaccinating children against flu helps protect wider community Results of a clinical trial conducted in a largely self-contained religious community during the 2008-09 influenza season show that immunizing children against seasonal influenza can... Surprising findings about Hepatitis C and insulin resistance We have known for several years that Hepatitis C, a common cause of liver cirrhosis and cancer, also makes people three to four times more... Starting treatment early doubles chance of success for people with diabetes The sooner people with diabetes start taking metformin, the longer the drug remains effective, according to a Kaiser Permanente study published in the March issue... Scientists discover 'catastrophic event' behind the halt of star birth in early galaxy formation Scientists have found evidence of a catastrophic event they believe was responsible for halting the birth of stars in a galaxy in the early Universe. Researchers develop new scale to measure anxiety outcomes A new questionnaire and outcomes measurement scale developed by the department of psychiatry at Rhode Island Hospital has proven to be a reliable and valid... Most extreme white dwarf binary system found with orbit of just 5 minutes An international team of astronomers, including Professor Tom Marsh and Dr. Danny Steeghs from the University of Warwick, have shown that the two stars in... Immigrants with disabilities more frequently employed than US-born persons with disabilities As the immigrant population grows, understanding its disability status and employment characteristics becomes increasingly important. People, both native and foreign-born, with disabilities make important contributions... Physician calls for more rigorous standards for drugs up for FDA approval In a commentary in JAMA, hospitalist Alec B. O'Connor calls for more stringent Federal guidelines governing the approval of potential new drugs. He says the... Life is shorter for men, but sexually active life expectancy is longer At age 55, men can expect another 15 years of sexual activity, but women that age should expect less than 11 years, according to a... The CSIC presents the Archive of Mourning concerning the terrorist attacks in Madrid It is a project that has worked with hundreds of photographs, drawings, texts and banners, among other materials placed at makeshift altars set up in... Better health associated with more frequent and better quality sex in later life People who are in good health are almost twice as likely to be interested in sex compared to those in poor health, according to research... Decoding the long calls of the orangutan Research into the long calls of male orangutans in Borneo has given scientists new insight into how these solitary apes communicate through dense jungle. An... Behavioral problems in childhood doubles the risk of chronic widespread pain in adult life Bad behavior in childhood is associated with long-term, chronic widespread pain in adult life, according to the findings of a study following nearly 20,000 people... Genetic variant greatly increases lung cancer risk for light smokers Individuals with a certain type of genetic susceptibility to lung cancer face a greatly increased risk for the deadly disease with even a small exposure... Skin transplant offers new hope to vitiligo patients In the first study of its kind in the United States, Henry Ford Hospital showed that skin transplant surgery is safe and effective for treating... Studies that compare effectiveness of medications often do not include nonpharmacologic therapies An analysis of comparative effectiveness studies finds that few compare medications with nonpharmacologic interventions, and few examine safety or cost-effectiveness, according to a study in... Donating kidney does not appear to significantly increase long-term risk of death An analysis of outcomes for live kidney donors in the US over a 15 year period finds they have similar long-term survival rates compared to... Vaccinating children for flu may help prevent transmission, protect those who are not vaccinated Immunizing children and adolescents with inactivated influenza vaccine resulted in reduced rates of influenza in their community compared to a similar community in which children... Kidney donors suffer few ill effects from life-giving act, landmark study finds In a landmark study of more than 80,000 live kidney donors from across the United States, Johns Hopkins researchers have found the procedure carries very... Elevated levels of cobalt and chromium found in offspring of moms with metal-on-metal hip implants Women with metal-on-metal hip implants, where both the ball of the joint and the surface of the socket are made of metal, pass metal ions... You have your MoM's ions Hip replacement patients with metal-on-metal implants (both the socket and hip ball are metal) pass metal ions to their infants during pregnancy, according to a... Study suggests need for broader use of individualized learning plans for physicians Physicians would be better prepared for the accelerating rate of scientific discovery -- and more in step with the latest in patient-care -- if they... Cleveland researchers use natural and artificial sheaths to mend traumatic bone loss Melissa Knothe Tate, of Case Western Reserve University, and Ulf Knothe, of the Cleveland Clinic, have shown that the stem-cell rich periosteum sheath around bone... Reovirus may be a novel approach to prostate cancer treatment Researchers in Canada have detected a novel oncolytic viral therapy against prostate cancer with use of a virus called the reovirus, according to study results... Obesity linked to poor colon cancer prognosis Obese patients with colon cancer are at greater risk for death or recurrent disease compared to those who are within a normal weight range, according... A full house raises risk of hospital deaths A full house isn't always a good thing, according to a new study by the University of Michigan Health System that links high hospital occupancy... New method to grow arteries could lead to 'biological bypass' for heart disease A new method of growing arteries could lead to a "biological bypass"-- or a noninvasive way to treat coronary artery disease, Yale School of Medicine... New study reveals prevalence of cyberbullying and its psychological impact on nonheterosexual youth According to a new national study by Iowa State University researchers, one out of every two lesbian, gay, bisexual, transgender (LGBT) and allied youths are... Exposure to BPA may cause permanent fertility defects, Yale researchers find Researchers at Yale School of Medicine have discovered that exposure during pregnancy to Bisphenol A (BPA), a common component of plastics, causes permanent abnormalities in... Like little golden assassins, 'smart' nanoparticles identify, target and kill cancer cells Another weapon in the arsenal against cancer: nanoparticles that identify, target and kill specific cancer cells while leaving healthy cells alone. AGU Journal highlights -- March 8, 2010 Featured in this release are research papers on the following topics: Lack of arches doubled Arctic strait's sea-ice loss; Solar wind pulses help blow away... Program could help teens control asthma An asthma program specifically tailored to teens could help those in rural areas manage their disease and avoid potentially fatal complications, Medical College of Georgia... Navy scientists support Haiti relief operations Scientists at the Naval Research Laboratory at Stennis Space Center are supporting Haiti relief operations. The Marine Geosciences Division's Geospatial Sciences and Technology branch... Americans want Uncle Sam's help putting healthy foods on their dinner table Americans recognize things need to change in the grocery aisle, and they support Uncle Sam's efforts to overhaul what is included in their food and... Musk ox population decline due to climate, not to humans, study finds Scientists have discovered that the drastic decline in Arctic musk ox populations that began roughly 12,000 years ago was due to a warming climate rather... MRI finds tumors in second breast of women diagnosed with cancer in one breast, Mayo researchers say Postmenopausal women, including those over 70 years old, who have been newly diagnosed with cancer in one breast have higher cancer detection rates when the... Study shows potential for using algae to produce human therapeutic proteins Pharmaceutical companies could substantially reduce the expense of costly treatments for cancer and other diseases produced from mammalian or bacterial cells by growing these human... Anti-depressants bring higher risk of developing cataracts: UBC-Vancouver Coastal Health research Some anti-depressant drugs are associated with an increased chance of developing cataracts, according to a new statistical study by researchers at the University of British... A possible early glimpse of autism's impact on older siblings A new study suggests a trend toward developing hyperactivity among typically developing elementary-school-aged siblings of autistic preschoolers and supports the notion that mothers of young,... Geraniums could help control devastating Japanese beetle Geraniums may hold the key to controlling the devastating Japanese beetle, which feeds on nearly 300 plant species and costs the ornamental plant industry $450... The influence of a romantic breakup on self-concept When a romantic relationship ends, an individual's self-concept is vulnerable to change, according to research in the February issue of Personality and Social Psychology Bulletin... LED streetlights best buy for cities, Pitt researchers report in first cradle-to-grave comparison to common and emerging streetlamps University of Pittsburgh researchers have conducted the first cradle-to-grave assessment of light-emitting diode streetlights and determined that the increasingly popular lamps strike the best balance... Gene discovered for newly recognized disease in Amish children The identification of the new multisystem autoimmune disorder and the recessive gene that causes it are reported in the March 2010 issue of American Journal... How to see through opaque materials New experiments show that it's possible to focus light through opaque materials and detect objects hidden behind them, provided you know enough about the material. Violent crime 'race gap' narrows, but persists in US The "race gap" in the commission of violent crime has narrowed substantially yet persists, with murder arrest rates for African-Americans out-distancing those for whites --... 'Improved doctor-pharmacist collaboration needed': study A research team from the Laval Centre de sante et de services sociaux, Universite de Montreal and McGill University Health Centre has examined the benefits... University of Michigan scientists discover bone marrow can harbor HIV-infected cells Antiviral drugs have reduced AIDS to more of chronic disease rather than a death sentence, but why is the disease so hard to cure? A... New ways to disarm deadly South American hemorrhagic fever viruses Researchers have discovered how one New World hemorrhagic fever virus latches onto and infects human cells, offering a much-needed lead toward new treatments. Asexual plant reproduction may seed new approach for agriculture An HHMI scientist has moved a step closer to turning sexually reproducing plants into asexual reproducers, a finding that could have profound implications for agriculture. Researchers show how far South American cities moved in quake The massive magnitude 8.8 earthquake that struck the west coast of Chile last month moved the entire city of Concepcion at least 10 feet to... Nutrition services for older adults at home and in communities The Society for Nutrition Education has partnered with the American Dietetic Association and American Society for Nutrition to publish a position paper focusing on access... Unhappy customers: Everyone has a right to complain, and does We've all had that sinking feeling when we got home and a purchase turned out to be damaged, or worse yet, we had no warranty... Infectious virus hidden in chromosomes during latency can be passed from parents to children In some individuals the common herpes virus HHV-6 can integrate into structures at the end of chromosomes and be reactivated to an infectious form. The... Is prenatal screening for rare diseases like spinal muscular atrophy too costly? Spinal Muscular Atrophy affects approximately 1 in 10,000 live births and is the leading genetic cause of infant mortality and the second most common autosomal... Immune cells use bungee of death to kill dangerous cells, shows new research Immune cells ensnare dangerous cells that are on the run with a bungee-like nanotube, according to research published today in the Proceedings of the National... Dietary supplements discouraged for prostate cancer patients Prostate-specific dietary supplements should not be taken during radiation therapy treatments because they have been shown to increase the radiosensitivity of normal prostate cell lines,... The truth about online dating and the link between depression and relational uncertainty There's no doubt that meeting partners on the Internet is a growing trend. But can we trust the information that people provide about themselves via... New research advances voice security technology Most people are familiar with security technology that scans a person's handprint or eye for identification purposes. Now, thanks in part to research from North... Deep sedimentation of acantharian cysts -- a reproductive strategy? Spore-like reproductive cysts of enigmatic organisms called acantharians rapidly sink from surface waters to the deep ocean in certain regions, according to new research. Scientists... NYU, U. of Wurzburg scientists shed new light on how retina's hardware is used in color vision Biologists at New York University and the University of Würzburg have identified, in greater detail, how the retina's cellular hardware is used in color preference.... New approach to immune cell analysis seen as first step to better distinguish health and disease Investigators have developed a new mathematical approach to analyze molecular data derived from complex mixtures of immune cells. This approach, when combined with well-established techniques,... Spotlight on rare tumors in hunt for new cancer treatments New breakthrough treatments for the most common cancers could soon come from cutting-edge research into some of the world's rarest tumors.At the ESMO Conference on... Two-faced testosterone can make you nasty or nice Is aggression always the best response to a challenge? Testosterone may not necessarily cause aggression but behavior can drive testosterone secretion. In an evaluation for... Strength is shore thing for sea shell scientists Scientists have made synthetic 'sea shells' from a mixture of chalk and polystyrene cups -- and produced a tough new material that could make our... The life and death of online communities The more heterogeneous the community of an online chat channel, the more chances the channel has to survive over time. 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