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Register Standards Bioinformatics Standards Flow Cytometry Standard (FCS) 3.0 Proposal Flow Cytometry Standard (FCS) 3.0 MIFlowCyt 1.0 MIFlowCyt 1.0 Summary Analytical Cytometry Standard NetCDF Analytical Cytometry Standard Gating-ML Proposal Analytical Cytometry Standard Gating-ML (full) Cytometry Standards Continuum Protocols Manufacturer Protocols Invitrogen BD Biosciences Biocompare - Flow Cytometry Web Available Cell Cycle Analysis BrDU and PI Staining Yeast Cell Cycle Fixation and DNA CFDA-SE for cell tracking and cell cycle analysis Collecting Mouse Tissue Samples FACS Staining Protocols Standard FACS Surface Staining One and Two Color Methods Surface and Intracellular Whole Blood Intracellular (by IntraCyte) Intracellular Cytokine Human Antibody Titiration MCB Staining Mario\'s Conjugation Hemoglobin ELISA Staining Protocols Intracellular Antigen Propidium Idodide Staining of Dead Cells Minimizing Background Staining Monoclonal Antibody Staining Staining Procedure for Whole Blood 7-AAD Staining of Dead Cells Freezing and Thawing PBMC MACS Depletion Protocol Preparation of 2% Formaldehyde Hawley&Hawley FC Protocols 2nd Ed. Multiparameter Flow Cytometry of Bacteria Multiparameter Data Acquisition and Analysis of Leukocytes Flow Cytometric Analysis of Kinase Signaling Cascades Cytokine Flow Cytometry Use of Cell-Tracking Dyes in Frequencies of Antigen Specific T-Cells Lymphocyte-Mediated Cytotoxicity Measurement Simultaneous- Cell Surface IF, Viability and DNA Simultaneous- Dual-Color IF and DNA/RNA Content Simutaneous- Three-Color IF and DNA Content Simultaneous- Apoptosis and Necrosis GFP Expression and DNA Content in Permeabilized Cells GFP Expression and DNA Content in Unfixed Cells Intracellular Ca2+ and Cell Curface Antigen Expression Parafomaldehyde Preparation Preparation of PBMC from Buffy Coats Antibody Labeling Fluorescein Isothiocyanate (FITC) Long Armed Biotin Texas Red Fluorescein Labeling Phycobiliproteins Hapten-anti-hapten labeling Immunolabeling of stable cell nuclei by flow cytometry Fluorochrome conjugation by amine modification Tietze Analysis Immunofluorescence Direct Direct IF Direct IF Basic Direct (by abcam) Indirect Indirect IF Basic Indirect (by abcam) DNA Analysis of Human Cancer Cell Lines NTS Buffer/DAPI Stain Content Analysis Using Hoechst 33342 in Unfixed Cells Blocking FC-Receptor Binding Detection of calcium flux with indo-1 Cell Death/Apoptosis Cell cycle and apoptosis analysis using DNA binding dyes Cell death analysis using PhiPhiLux caspase 3 substrate and annexin V Detection of Apoptosis by 7-AAD Staining Current Protocols in Cytometry (Interscience Wiley) Educational Online Texts Practical Flow Cytometry 4th Ed., Shapiro (2005) Flow Cytometry Protocols 2nd Ed., Hawley & Hawley (2004) Cytometry 3rd Ed. Vol. 63, Darzynkiewicz Flow Cytometry Protocols, Jaroszeski and Heller (1998) Flow Cytometry for Biotechnology, Sklar (2005) Flow Cytometry with Plant Cells. Dolezel, Greilhuber and Suda (2007) Flow Cytometry: A Practical Approach, Ormerod (2003) Cytometric Analysis of Cell Phenotype and Function, McCarthy and Macey, (2003) Flow Cytometry: Principles and Applications, Macey (2007) Flow Cytometry in Hematopahtology, Nguyen, Diamond and Braylan, (2003) Introduction to Flow Cytometry Flow Cytometry Data Analysis Techniques in Cell Cycle Analysis Fluorochrome Tables Salk Institute Table Invitrogen Table Sciencegateway Table BD Biosciences Viewer Cytometry Tutorials and Presentations Video Tutorials Compensation Pt. 1 (Marty Bigos) Compensation Pt. 2 (Mario Roderer) Compensation Pt. 3 (Nicole Baumgarth) Cytometry Overviews by Invitrogen by BD Biosciences by J. 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RSS Reality TV, cosmetic surgey linked, says Rutgers-Camden researcher Research by a Rutgers-Camden psychologist suggests that teens fond of reality TV programs are more likely to join the millions who go under the knife... Reading terrorists minds about imminent attack Imagine technology that allows you to get inside the mind of a terrorist to know how, when and where the next attack will occur. That... Some like it hot: How to heat a 'nano bathtub' the JILA way Researchers at JILA have demonstrated the use of infrared laser light to quickly and precisely heat the water in "nano bathtubs" -- tiny sample containers... Oral contraceptives and hormone replacement therapy may protect women against brain aneurysms Results from a new study suggest that oral contraceptives and hormone replacement therapy may yield additional benefit of protecting against the formation and rupture of... Body of evidence: New fast, reliable method to detect gravesoil Finding bodies buried by someone who wanted them to stay undiscovered can be difficult. However a new technique developed by scientists at the National Institute... Nano 'pin art': NIST arrays are step toward mass production of nanowires Researchers at the National Institute of Standards and Technology have cultivated many thousands of nanocrystals in what looks like a pinscreen or "pin art" on... Breaking the language barrier: NIST tests language translation devices for US troops In recent tests evoking visions of the universal translator on "Star Trek," NIST evaluated three two-way, real-time, voice-translation devices designed to improve communications between the... Emerging E. coli strain causes many antimicrobial-resistant infections in US A new, drug-resistant strain of E. coli is causing serious disease, according to a new study, now available online, in the Aug. 1, 2010, issue... Kinked nanopores slow DNA passage for easier sequencing In an innovation critical to improved DNA sequencing, a markedly slower transmission of DNA through nanopores has been achieved by a team led by Sandia... Do soy isoflavones boost bone health? Scientists already know much about the more than 200 bones that make up your body. But mysteries remain regarding the exact role that many natural... New theory of why midcontinent faults produce earthquakes A new theory developed at Purdue University may solve the mystery of why the New Madrid fault, which lies in the middle of the continent... Pilot study supports adolescent diabetes patients through personalized text messages Jennifer Dyer, M.D., M.P.H., an endocrinologist at Nationwide Children's Hospital, has developed and completed a pilot study that uses weekly, customized text messages to remind... 'Path of mental illness' follows path of war, 20 years after conflict ends Researchers at Columbia University's Mailman School of Public Health assessed the geographical distribution of the long-term burden of post-traumatic stress disorder (PTSD) in a region... From the heart: How cells divide to form different but related muscle groups Using the model organism Ciona intestinalis, commonly known as the sea squirt, researchers at the University of California, Berkeley, have uncovered the origins of the... New approach to Alzheimer's therapy Researchers from the German Centre for Neurodegenerative Diseases and the Ludwig-Maximilians-Universitat in Munich have shown that the ADAM10 protein can inhibit the formation of beta-amyloid,... One more step on the path to quantum computers Researchers around the world are working on the development of quantum computers that will be vastly superior to present-day computers. Here, the strong coupling of... Research of cell movements in developing frogs reveals new twists in human genetic disease Mutations in a gene known as "Fritz" may be responsible for causing human genetic disorders such as Bardet-Biedl syndrome, University of Texas at Austin developmental... Study finds diet and alcohol alter epigenetics of breast cancer Researchers from Brown University and the University of California have shown that the epigenetic profiles of breast tumors are related to patient diet and alcohol... New 'armor' developed to avoid infection from AIDS virus The doors are closing on the AIDS virus. A study by the Consejo Superior de Investigaciones Científicas has developed a method of attack against the... Reforestation projects capture more carbon than industrial plantations, reveals new research Australian scientists researching environmental restoration projects have found that the reforestation of damaged rainforests is more efficient at capturing carbon than controversial softwood monoculture plantations.... A potential chemotherapeutic drug to treat hepatocellular carcinoma A research team from China investigated the effect of galangin on hepatocellular carcinoma (HCC) cells. They found that galangin mediates apoptosis through a mitochondrial pathway,... Vanishing bile duct syndrome secondary to anti-retroviral therapy in HIV Vanishing bile duct syndrome (VBDS) is an important cause of jaundice, and results from destruction of bile ducts in the liver. However, this syndrome is... How to detect malnutrition in patients effectively? Malnutrition is a common problem in patients with cancer and is associated with a poor outcome. The assessment of nutritional status and its evaluation plays... Effective inducing systems of hepatic differentiation from bone marrow mesenchymal stem cells Mesenchymal stem cells (MSCs) are multipotent non-hematopoietic cells capable of differentiating into hepatocytes. Valproic acid (VPA), a histone deacetylase inhibitor, has recently exhibited profound therapeutic... CD74 serves as a survival receptor on colon epithelial cells CD74 is a protein expressed by cells of the immune system. A research group in Israel finds that CD74 is expressed on colon epithelial cells... Functional magnetic resonance imaging to evaluate pancreatic cancer In an effort to develop new therapies for pancreatic cancer, models of this disease must be created and characterized. A recent study from United States... New study: Tools that assess bias in standardized tests are flawed Overturning more than 40 years of accepted practice, new research proves that the tools used to check tests of "general mental ability" for bias are... 30 million women to benefit from health reform law Thirty million women will benefit from the new health reform law over the next decade, either through new or strengthened insurance coverage, according to a... Clinical trials can be improved by managing the learning curve Researchers at Duke University Medical Center looked for a learning curve phenomenon in the data record of a large, multi-site clinical trial. Their findings point... Transforaminal steroid injection for lumbar radicular pain proves superior to placebo A recent study from Australian researchers determined that transforaminal injection of steroids was a viable alternative to surgery for lumbar radicular pain due to disc... Intervention effort cuts HIV incidence among female sex workers A team of researchers from the University of California San Diego and Mexico has found that even a modest behavioral intervention program averaging just 35... University of Nevada, Reno tests cutting-edge technology for underwater mapping at Tahoe basin A borrowed boat, a small mountain lake and the inaugural run of a half-a-million dollar state-of-the-art multi-beam sonar system made history this month with the... UC Davis study finds worse mental health associated with worse pain in osteoarthritis How much pain osteoarthritis sufferers feel is directly related to their mental health, a new study by researchers at UC Davis School of Medicine has... Decontaminating dangerous drywall A nanomaterial originally developed to fight toxic waste is now helping reduce debilitating fumes in homes with corrosive drywall. Calcium supplements play an important role in maintaining bone health A broad range of scientific research has demonstrated that an adequate intake of calcium plays an important role in building and maintaining optimum bone mass,... If spiders and worms can do it, why can't we? Imagine a material that is tougher than Kelvar or steel, yet remarkably flexible. It's something you can easily find in your attic or a lingerie... UCI immunological study finds earlier way to diagnose axon and neuron degeneration in MS UCI immunologists have found that testing for increased levels of antibodies that inhibit energy production in neurons can detect axon and neuron degeneration in multiple... Snake venom studies yield insights for development of therapies for heart disease and cancer Researchers seeking to learn more about stroke by studying how the body responds to toxins in snake venom are this week releasing new findings that... Story tips from the Department of Energy's Oak Ridge National Laboratory, August 2010 The following are story tips from the US Department of Energy's Oak Ridge National Laboratory for August 2010. Mechanism uncovered behind Salmonella virulence and drug susceptibility Researchers have discovered a novel mechanism in Salmonella that affects is virulence and its susceptibility to antibiotics. The mechanism changes the bacteria's production of proteins... Resting brain activity associated with spontaneous fibromyalgia pain A recent study from researchers at Massachusetts General Hospital and University of Michigan provides the first direct evidence of linkage between elevated intrinsic (resting-state) brain... Scientists post lower speed limit for cell-signaling protein assembly The apparently random self-assembly of molecular threads into the proteins that make the body work is far less frantic than previously thought, Michigan State University... TNF blockers may increase the risk of malignancy in children The US Food and Drug Administration received reports of malignancies in children using tumor necrosis factor a (TNF) blockers, raising concerns of an associated risk... Brown dwarf found orbiting a young sun-like star Astronomers have imaged a very young brown dwarf, or failed star, in a tight orbit around a young nearby sun-like star. The discovery is expected... Polymer passage takes time Rice University researchers have created a theoretical method to calculate the time it takes long-chain polymers to pass through nano-sized pores in membranes. The researchers... Some trees 'farm' bacteria to help supply nutrients Some trees growing in nutrient-poor forest soil may get what they need by cultivating specific root microbes to create compounds they require. These microbes are... Graphene under strain creates gigantic pseudo-magnetic fields By putting the right kind of strain onto a patch of graphene, Berkeley Lab researchers have created pseudo-magnetic fields far stronger than the strongest magnetic... Study finds black carbon implicated in global warming Increasing the ratio of black carbon to sulfate in the atmosphere increases climate warming, suggests a study conducted by a University of Iowa professor and... Memory's master switch A new study led by Dr. Inna Slutsky of Tel Aviv University describes GABA (γ-Aminobutyric acid), a natural molecule that occurs in the brain, which... Most youth hockey injuries caused by accidents, not checking, UB study shows Hockey fans likely would assume that body-checking -- intentionally slamming an opponent against the boards -- causes the most injuries in youth ice hockey. But... Behind the secrets of silk lie high-tech opportunities Tougher than a bullet-proof vest yet synonymous with beauty and luxury, silks spun by worms and spiders are a masterpiece of nature whose properties have... Plant compound resveratrol shown to suppresses inflammation, free radicals in humans Resveratrol, a popular plant extract shown to prolong life in yeast and lower animals due to its anti-inflammatory and antioxidant properties, appears also to suppress... New pathway to Parkinson's and Alzheimer's diseases Sanford-Burnham researchers uncover new clues about the cause of brain cell death in neurodegenerative disorders such as Parkinson's, Alzheimer's and Huntington's diseases. Ear bones reveal spawning secrets of Lake Erie walleye Ecologists have long believed that fish tend to return to the same river where they hatched in order to spawn. But researchers at Ohio State... Discovered: Audubon's first engraving of a bird In 1824, John James Audubon (1785-1851), the eminent American artist, created a drawing of a running grouse for use in the design for a New... 'White graphene' to the rescue Researchers in the lab of Pulickel Ajayan, Rice's Benjamin M. and Mary Greenwood Anderson Professor in Mechanical Engineering and Materials Science and of chemistry, have... Male breast cancer in family leads to high perception of risk, low likelihood of genetic counseling People with a family history of male breast cancer perceive themselves to be at higher risk of developing the disease than do patients with a... Carnegie Mellon researchers create fluorescent biosensor to aid in drug development Carnegie Mellon University has developed a new fluorescent biosensor that could aid in the development of an important class of drugs that target a crucial... Study finds respiratory symptoms more reliable indicator of H1N1, not fever alone New research shows that individuals with mild H1N1 infection may go undetected using standard diagnostic criteria, according to a study in the August issue of... Gender-bending fish on the rise in southern Alberta Chemicals present in two rivers in southern Alberta are likely the cause of the feminization of fish say researchers at the University of Calgary who... Rutgers study finds male modesty a turn off for women (and men) A Rutgers researcher who explored the consequences for men (and women) when they acted modestly in job interviews found that "modest" males were less liked,... Researchers identify key enzyme in DNA repair pathway Researchers have discovered an enzyme crucial to a type of DNA repair that also causes resistance to a class of cancer drugs most commonly used... A new ground zero for prostate cancer A type of prostate cell that has been largely ignored by cancer researchers can trigger malignant prostate cancer. The studies provide researchers with a new... The evolution of melanoma diagnosis: 25 years beyond the ABCDs Twenty-five years after publishing the mnemonic "ABCD" to facilitate the early diagnosis of melanoma, the group who came up with that moniker says early detection... To make one happy, make one busy A new study in Psychological Science found that people who have something to do, even something pointless, are happier than people who sit idly. More frequent, more intense heat waves in store for New York Heat waves like those that baked the Northeast in July are likely to be more frequent and more intense in the future, with their effects... Rocks on Mars may provide link to evidence of living organisms roughly 4 billion years ago A new article in press of the journal Earth and Planetary Science Letters unveils groundbreaking research on the hydrothermal formation of clay-carbonate rocks in the... Highlight of the Polarstern expedition The Alfred Wegener Institute for Polar and Marine Research in the Helmholtz Association for the first time sent its autonomous underwater vehicle on an under-ice... Researchers study benefits of white button mushrooms Agricultural Research Service-funded scientists have conducted an animal-model and cell-culture study showing that white button mushrooms enhanced the activity of critical cells in the body's... The thunderstone mystery Archeologists Olle Hemdorff og Eva Thäte at the University of Stavanger's Museum of Archaeology investigate finds of older artifacts in younger graves. They have found... Signs of reversal of Arctic cooling in some areas Parts of the Arctic have cooled clearly over the past century, but temperatures have been rising steeply since 1990 also there. This is the finding... Good and bad in the hands of politicians "In laboratory tests, right- and left-handers associate positive ideas like honesty and intelligence with their dominant side of space and negative ideas with their nondominant... Small increases in vaccine cost can cause large gaps in protection Public immunization efforts may be much more sensitive than previously realized to small changes in the perceived costs or risks of vaccination, scientists at Harvard... A breakthrough in tuberculosis research The key to the fate of the macrophages is the balance between two kinds of eicosanoids. Eicosanoids are molecules that contribute to the control of... Western diet link to ADHD A new study from Perth's Telethon Institute for Child Health Research shows an association between ADHD and a "Western-style" diet in adolescents. The research findings... Calcium supplements linked to increased risk of heart attack Calcium supplements, commonly taken by older people for osteoporosis, are associated with an increased risk of a heart attack, finds a study published on bmj.com... Empa grows 'sea urchin'-shaped structures Empa researchers have succeeded in growing sea-urchin shaped nanostructures from minute balls of polystyrene beads using a simple electrochemical process. The spines of the sea... 'Linc-ing' a noncoding RNA to a central cellular pathway The recent discovery of more than a thousand genes known as large intergenic noncoding RNAs (or "lincRNAs") opened up a new approach to understanding the... Brain potentials reveal spectator effect The neurological responses caused by observing somebody else playing a game have been uncovered. Researchers writing in the open access journal BMC Neuroscience found differing... Ancient reptiles 'make tracks' A new discovery of fossilized footprints reveals when reptiles first conquered dry land. A leap forward in addiction awareness and control A study by a team of researchers at Bangor University has designed and tested two programs that help problem drinkers curb their alcohol abuse. The... Researchers find new translocation; weak spots in DNA lead to genetic disease A pediatric research team at the Children's Hospital of Philadelphia continues to discover recurrent translocations -- places in which two chromosomes exchange pieces of themselves,... Popular diabetes drugs associated with fractures in type 2 diabetic patients Postmenopausal women with diabetes taking thiazolidinediones (TZDS), including rosiglitazone and pioglitazone, may be at increased risk for fractures according to a new study accepted for... Vitamin D deficiency linked to arterial stiffness in black teens Vitamin D deficiency is associated with arterial stiffness, a risk factor for heart disease and stroke, in black teens according to a new study accepted... Unexpected viral 'fossils' found in vertebrate genomes Over millions of years, retroviruses, which insert their genetic material into the host genome as part of their replication, have left behind bits of their... Just drop it: The one-size-fits-all approach to blood sugar control, that is Aggressive blood sugar control does not improve survival in diabetic patients with kidney failure, according to a study appearing in an upcoming issue of the... Kidney injury prevention may be possible: Watch for the warning signs Reduced kidney function and protein in the urine place a person at risk for kidney injury, according to a study appearing in an upcoming issue... Graphene exhibits bizarre new behavior well suited to electronic devices Graphene, a sheet of pure carbon, has been touted as a possible replacement for silicon-based semiconductors because of its useful electronic properties. Now, UC Berkeley... UCLA scientists for the first time identify a cell-of-origin for human prostate cancer UCLA scientists have identified for the first time a cell-of-origin for human prostate cancer, a discovery that could result in better predictive and diagnostics tools... Next generation sequencing establishes genetic link between two rare diseases Scientists have successfully used "next generation sequencing" to identify mutations that may cause a rare and mysterious genetic disorder. The research, published by Cell Press... New lab test could identify imatinib resistance Scientists in Japan may have developed a way to accurately predict those patients who will resist treatment with imatinib, which is the standard of care... Quality coronary bypass care can improve lives and cut costs A new analysis led by researchers at UCSF shows that avoiding lowest-volume hospitals and maximizing adherence to quality care processes are both effective approaches to... Super-rare 'elkhorn' coral found in Pacific An Australian scientist has discovered what could be the world's rarest coral in the remote North Pacific Ocean. The unique Pacific elkhorn coral was found... Groundbreaking research from Intel Corp. demonstrated at IPR Groundbreaking research presented by Intel OSA topical meeting, Integrated Photonics Research, Silicon and Nano Photonics (IPR). IPR is currently being held at the Monterey Plaza... Intensive chemotherapy may be harmful to most older patients with acute myeloid leukemia The prognosis for nearly three-quarters of elderly patients on intensive chemotherapy for acute myeloid leukemia is poor, with a median survival of less than six... Scripps research study opens the door to new class of drugs for epileptic seizures A chemical compound that boosts the action of a molecule normally produced in the brain may provide the starting point for a new line of... Quantum fractals at the border of magnetism Physicists from Rice University, the Max Planck Institute and the Vienna University of Technology are reporting new results from experiments on the perplexing class of... Nanomaterials poised for big impact in construction Nanomaterials are poised for widespread use in the construction industry, where they can offer significant advantages for a variety of applications ranging from making more... Brainstem, spinal cord images hidden in Michelangelo's Sistine Chapel fresco Michelangelo, the 16th century master painter and accomplished anatomist, appears to have hidden an image of the brainstem and spinal cord in a depiction of... Dense bones linked to raised risk for prostate cancer Men who develop prostate cancer, especially the more aggressive and dangerous forms that spread throughout the body, tend to retain denser bones as they age...   [ Back ] News Feeds and Abstracts ISAC Updates and News Cytometry Part A Abstracts Cytometry Part B Abstracts Plant Flow Cytometry Blog Daily Dongle Feed Maekerlab Abstract Digest FlowCytometryNews.net EurekAlert! 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