Home Contact Us   Top Menu Standards Bioinformatics Standards Flow Cytometry Standard (FCS) 3.0 Proposal Flow Cytometry Standard (FCS) 3.0 MIFlowCyt 1.0 MIFlowCyt 1.0 Summary Analytical Cytometry Standard NetCDF Analytical Cytometry Standard Gating-ML Proposal Analytical Cytometry Standard Gating-ML (full) Cytometry Standards Continuum Protocols Manufacturer Protocols Invitrogen BD Biosciences Biocompare - Flow Cytometry Web Available Cell Cycle Analysis BrDU and PI Staining Yeast Cell Cycle Fixation and DNA CFDA-SE for cell tracking and cell cycle analysis Collecting Mouse Tissue Samples FACS Staining Protocols Standard FACS Surface Staining One and Two Color Methods Surface and Intracellular Whole Blood Intracellular (by IntraCyte) Intracellular Cytokine Human Antibody Titiration MCB Staining Mario\'s Conjugation Hemoglobin ELISA Staining Protocols Intracellular Antigen Propidium Idodide Staining of Dead Cells Minimizing Background Staining Monoclonal Antibody Staining Staining Procedure for Whole Blood 7-AAD Staining of Dead Cells Freezing and Thawing PBMC MACS Depletion Protocol Preparation of 2% Formaldehyde Hawley&Hawley FC Protocols 2nd Ed. Multiparameter Flow Cytometry of Bacteria Multiparameter Data Acquisition and Analysis of Leukocytes Flow Cytometric Analysis of Kinase Signaling Cascades Cytokine Flow Cytometry Use of Cell-Tracking Dyes in Frequencies of Antigen Specific T-Cells Lymphocyte-Mediated Cytotoxicity Measurement Simultaneous- Cell Surface IF, Viability and DNA Simultaneous- Dual-Color IF and DNA/RNA Content Simutaneous- Three-Color IF and DNA Content Simultaneous- Apoptosis and Necrosis GFP Expression and DNA Content in Permeabilized Cells GFP Expression and DNA Content in Unfixed Cells Intracellular Ca2+ and Cell Curface Antigen Expression Parafomaldehyde Preparation Preparation of PBMC from Buffy Coats Antibody Labeling Fluorescein Isothiocyanate (FITC) Long Armed Biotin Texas Red Fluorescein Labeling Phycobiliproteins Hapten-anti-hapten labeling Immunolabeling of stable cell nuclei by flow cytometry Fluorochrome conjugation by amine modification Tietze Analysis Immunofluorescence Direct Direct IF Direct IF Basic Direct (by abcam) Indirect Indirect IF Basic Indirect (by abcam) DNA Analysis of Human Cancer Cell Lines NTS Buffer/DAPI Stain Content Analysis Using Hoechst 33342 in Unfixed Cells Blocking FC-Receptor Binding Detection of calcium flux with indo-1 Cell Death/Apoptosis Cell cycle and apoptosis analysis using DNA binding dyes Cell death analysis using PhiPhiLux caspase 3 substrate and annexin V Detection of Apoptosis by 7-AAD Staining Current Protocols in Cytometry (Interscience Wiley) Educational Online Texts Practical Flow Cytometry 4th Ed., Shapiro (2005) Flow Cytometry Protocols 2nd Ed., Hawley & Hawley (2004) Cytometry 3rd Ed. Vol. 63, Darzynkiewicz Flow Cytometry Protocols, Jaroszeski and Heller (1998) Flow Cytometry for Biotechnology, Sklar (2005) Flow Cytometry with Plant Cells. Dolezel, Greilhuber and Suda (2007) Flow Cytometry: A Practical Approach, Ormerod (2003) Cytometric Analysis of Cell Phenotype and Function, McCarthy and Macey, (2003) Flow Cytometry: Principles and Applications, Macey (2007) Flow Cytometry in Hematopahtology, Nguyen, Diamond and Braylan, (2003) Introduction to Flow Cytometry Flow Cytometry Data Analysis Techniques in Cell Cycle Analysis Fluorochrome Tables Salk Institute Table Invitrogen Table Sciencegateway Table BD Biosciences Viewer Cytometry Tutorials and Presentations Video Tutorials Compensation Pt. 1 (Marty Bigos) Compensation Pt. 2 (Mario Roderer) Compensation Pt. 3 (Nicole Baumgarth) Cytometry Overviews by Invitrogen by BD Biosciences by J. 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RSS Henry Ford Hospital researchers identity potential biomarker for osteoarthritis Henry Ford Hospital researchers have identified for the first time two molecules that hold promise as a biomarker for measuring cartilage damage associated with osteoarthritis.Researchers... Hearing metaphors activates brain regions involved in sensory experience New brain imaging research reveals that a region of the brain important for sensing texture through touch, the parietal operculum, is also activated when someone... Why 2 new studies represent important breakthrough in Alzheimer's disease research Two separate research findings have the potential to give us a much more sophisticated understanding of what goes wrong in Alzheimer's disease and what can... Whole exome sequencing identifies cause of metabolic disease Sequencing a patient's entire genome to discover the source of his or her disease is not routine, but geneticists are getting close. A case report... Study: Stroke victims recover much better after temporary stent procedure A new way of opening blocked arteries in the brain using a removable stent system in people suffering strokes brought remarkably positive results in how... Discovery of extremely long-lived proteins may provide insight into cell aging One of the big mysteries in biology is why cells age. Now scientists at the Salk Institute for Biological Studies report that they have discovered... Dieting with the denomination, determination According to a new study, those starting new weight loss programs may be surprised to find out that both location and level of experience may... Study published in Neuro-Oncology shows brain tumor eradication and prolonged survival Tocagen Inc. today announced the publication of data showing the company's investigational treatment for high grade glioma eradicates brain tumors and provides a dramatic survival... Regular use of vitamin and mineral supplements could reduce the risk of colon cancer Could the use of vitamin and mineral supplements in a regular diet help to reduce the risk of colon cancer and protect against carcinogens? A... Coughing and other respiratory symptoms improve within weeks of smoking cessation A new study shows that 18- to 24-year olds who stop smoking for at least two weeks report substantially fewer respiratory symptoms, especially coughing. The... New device removes stroke-causing blood clots better than standard treatment An experimental device for removing blood clots in stroke patients dramatically outperformed the standard mechanical treatment, according to research presented by UCLA Stroke Center director... UC San Diego Moores Cancer Center offers new hope for deadly brain tumor In the United States, each year, approximately 10,000 patients are affected by recurrant glioblastoma multiforme. Now, a novel investigational device - available only at... The complex relationship between memory and silence People who suffer a traumatic experience often don't talk about it, and many forget it over time. But not talking about something doesn't always mean... Schooling protects fleeing children from disease Refugee children have scant access to medical care and are particularly vulnerable to disease. Fresh research results from the University of Copenhagen show that just... A new study shows how to boost the power of pain relief, without drugs Placebos reduce pain by creating an expectation of relief. Distraction -- say, doing a puzzle -- relieves it by keeping the brain busy. But do... Scientists chart high-precision map of Milky Way's magnetic fields Scientists at the Naval Research Laboratory are part of an international team that has pooled their radio observations into a database, producing the highest precision... Warfarin and aspirin are similar in heart failure treatment In the largest and longest head-to-head comparison of two anti-clotting medications, warfarin and aspirin were similar in preventing deaths and strokes in heart failure patients... Rare mutations may help explain aneurysm in high-risk families An innovative approach to genome screening has provided clues about rare mutations that may make people susceptible to brain aneurysms, predisposing them to brain bleeds,... Vitamin D deficiency in geriatric patients The great majority of geriatric patients in a German rehabilitation hospital were found to have vitamin D deficiency. Stefan Schilling presents his study results in... New drug doesn't improve disability among stroke patients A new drug that showed promise in animal studies and an early clinical trial didn't improve disability among stroke patients, according to late-breaking research presented... Clopidogrel with aspirin doesn't prevent more small strokes, may increase risk of bleeding, death The anti-blood clot regimen that adds the drug clopidogrel (Plavix) to aspirin treatment is unlikely to prevent recurrent strokes and may increase the risk of... New device performs better than old for removing blood clots An experimental blood clot-removing device outperformed the FDA-approved MERCI; retriever device, according to late-breaking science presented at the American Stroke Association's 2012 International Stroke Conference. Castaway lizards offer new look at evolutionary processes Biologists who released lizards on tiny uninhabited islands in the Bahamas have uncovered a seldom-observed interaction between evolutionary processes.Jason Kolbe, a biologist at the University... Media portrayal of race in sports reveals biases in corporate world The US may have its first black president and the Fortune 500 its first black female chief executive, but African American CEOs account for a... Gene related to fat preferences in humans found A preference for fatty foods has a genetic basis, according to researchers, who discovered that people with certain forms of the CD36 gene may like... Lower levels of sunlight link to allergy and eczema Increased exposure to sunlight may reduce the risk of both food allergies and eczema in children, according to a new scientific study published this week. Dignity counts when caring for older people Older people feel that their health problems pose a challenge to their sense of independence, dignity and identity and sometimes the health care they are... Soy isoflavone supplements did not provide breast cancer protections Findings suggest the effects of food may be more complex. Adverse effect observed in younger women. Global extinction: Gradual doom is just as bad as abrupt Around 250 million years ago, most life on Earth was wiped out in an extinction known as the "Great Dying." A team led by University... Surface of Mars an unlikely place for life after 600 million year drought, say scientists Mars may have been arid for more than 600 million years, making it too hostile for any life to survive on the planet's surface, according... Judder-free videos on the smartphone Overloaded cellular networks can get annoying - especially when you want to watch a video on your smartphone. An optimized Radio Resource Manager will soon... Collective action Genetic switches called enhancers and the molecules that activate them can be used to draw a cell's family tree, EMBL scientists have found. Making sense of addiction terminology A new editorial released this week offers clarity and structure on confusing drug and alcohol addiction terminology for prescribers, users and regulators. Jointly utilizing LTE networks Data-intensive Internet applications on smartphones, tablets and laptops are more popular than ever before. The result: Traffic on the mobile network is increasing at a... Sediments from the Enol lake reveal more than 13,500 years of environmental history A team of Spanish researchers have used different geological samples, extracted from the Enol lake in Asturias, to show that the Holocene, a period that... Parasites or not? Transposable elements in fruit flies The problem of parasitism occurs at all levels right down to the DNA scale. Genomes may contain up to 80 percent "foreign" DNA but... NYU Courant researchers weigh methods to more accurately measure genome sequencing Researchers at New York University's Courant Institute of Mathematical Sciences evaluate some current methods to sequence individual genomes -- a study that serves as a... New technology to tackle treatment-resistant cancers Free-flowing cancer cells have been mapped with unprecedented accuracy in the bloodstream of patients with prostate, breast and pancreatic cancer, using a brand new approach,... New technique dissolves blood clots in the brain and lowers risk of brain damage after stroke Johns Hopkins neurologists report success with a new means of getting rid of potentially lethal blood clots in the brain safely without cutting through easily... New procedure repairs severed nerves in minutes, restoring limb use in days or weeks American scientists believe a new procedure to repair severed nerves could result in patients recovering in days or weeks, rather than months or years. The... Classic portrait of a barred spiral galaxy The NASA/ESA Hubble Space Telescope has taken a picture of the barred spiral galaxy NGC 1073, which is found in the constellation of Cetus (The... Breastfeeding and lung function at school age: Does maternal asthma modify the effect? Breastfeeding is associated with improved lung function at school age, particularly in children of asthmatic mothers, according to a new study from researchers in Switzerland... A battle of the vampires, 20 million years ago? They are tiny, ugly, disease-carrying little blood-suckers that most people have never seen or heard of, but a new discovery in a one-of-a-kind fossil shows... NASA satellites see wind shear battering Tropical Depression Iggy NASA satellites have watched as wind shear has torn Cyclone Iggy apart over the last day. NASA infrared satellite imagery showed that Iggy's strongest thunderstorms... 'First light' taken by NASA's newest CERES instrument The doors are open on NASA's Suomi NPP satellite and the newest version of the Clouds and the Earth's Radiant Energy System (CERES) instrument is... Hubble zooms in on a magnified galaxy Thanks to the presence of a natural "zoom lens" in space, NASA's Hubble Space Telescope got a uniquely close-up look at the brightest "magnified" galaxy... New RNA-based therapeutic strategies for controlling gene expression Small RNA-based nucleic acid drugs represent a promising new class of therapeutic agents for silencing abnormal or overactive disease-causing genes, and researchers have discovered new... Football findings suggest concussions caused by series of hits A two-year study of high school football players suggests that concussions are likely caused by many hits over time and not from a single blow... Scientists coax shy microorganisms to stand out in a crowd Scientists have advanced a method that allowed them to single out a marine microorganism and map its genome even though the organism made up less... Google Earth ocean terrain receives major update Internet information giant Google updated ocean data in its Google Earth application this week, reflecting new bathymetry data assembled by Scripps Institution of Oceanography, UC... Triglyceride levels predict stroke risk in postmenopausal women A new study by researchers at NYU Langone Medical Center and colleagues found that traditional risk factors for stroke -- such as high cholesterol --... Diabetes rates vary widely in developing countries, 1 in 10 cases untreated Rates of diabetes vary widely across developing countries worldwide, according to a new analysis led by Dr. Longjian Liu of Drexel University's School of Public... A zap of cold plasma reduces harmful bacteria on raw chicken in Drexel study A new study by food safety researchers at Drexel University demonstrates that plasma can be an effective method for killing pathogens on uncooked poultry. The... UAHuntsville business faculty investigate research ethics; Results are published in Science magazine Two UAHuntsville faculty members from the College of Business were published today in the prestigious journal Science for their investigation of an important issue in... New research confirms need for lung cancer testing Different kinds of lung cancer behave in different ways, suggesting they are fundamentally different diseases. According to a University of Colorado Cancer Center study published... Planets circling around twin suns Double suns -- stars that are formed as a pair -- are a common phenomenon in the cosmos. But now NASA, working with a Tel... Bouquet bargains Most creatures face compromises when they reproduce -- the more energy they devote to having lots of babies, the less they can invest in each... Best management practices for invasive crane flies in northeastern United States sod production A new study recently published in the Journal of Integrated Pest Management explains the best management practices for consideration and adoption by sod producers in... Male and female behavior deconstructed Now a team of scientists at the University of California, San Francisco has uncovered many genes influenced by the male and female sex hormones testosterone... High triglyceride levels found to predict stroke in older women In a surprising finding with significant implications for older women, researchers at Albert Einstein College of Medicine of Yeshiva University and NYU School of Medicine... Balancing oxaliplatin dose with neurological side effects in metastatic colon cancer "Stop-and-go" dosing along with calcium and magnesium supplements reduce neurological side effects of oxaliplatin use with metastatic colon cancer patients. Treasure trove of wildlife found in Peru park The Wildlife Conservation Society's Peru program announced today the discovery of 365 species previously undocumented in Bahuaja Sonene National Park in southeastern Peru. Untangling the mysteries of Alzheimer's Researchers have found new evidence that confirms the significance of a protein that neuroscientists call tau to the development of Alzheimer's disease. While earlier studies... DNA test that identifies Down syndrome in pregnancy can also detect trisomy 18 and trisomy 13 A recent study by Drs. Glenn Palomaki and Jacob Canick of Women & Infants Hospital shows that a new DNA test that identifies Down syndrome... Southampton research shows early bone growth linked to bone density in later life Researchers from the University of Southampton, in collaboration with a research group in Delhi, India, have shown that growth in early childhood can affect bone... The discovery of deceleration Stellar astrophysics helps to explain the behavior of fast rotating neutron stars in binary systems. Investigational urine test can predict high-risk prostate cancer in men who chose 'watchful waiting' Initial results of a multicenter study coordinated by researchers at Fred Hutchinson Cancer Research Center indicates that two investigational urine-based biomarkers are associated with prostate... Lecture or listen: When patients waver on meds According to a new analysis of hundreds of recorded office visits, doctors and nurse practitioners typically issued orders and asked closed or leading questions when... New super-Earth detected within the habitable zone of a nearby cool star An international team of scientists led by Carnegie's Guillem Anglada-Escudé and Paul Butler has discovered a potentially habitable super-Earth orbiting a nearby star. The star... Being confined to bed… Being confined to bed…can have fatal consequences. Incorrect fastening of restraints and inadequate monitoring led to the death of 19 people in care. Andrea M.... Studying butterfly flight to help build bug-size flying robots By figuring out how butterflies flutter among flowers with amazing grace and agility, researchers hope to help build small airborne robots that can mimic those... Elevated glucose associated with undetected heart damage A new study led by researchers at the Johns Hopkins Bloomberg School of Public Health suggests that hyperglycemia injures the heart, even in patients without... A new screening method for prostate cancer A new study by NYU Langone Medical Center and Northwestern University Feinberg School of Medicine shows novel PSA velocity risk count testing may provide a... Rituximab possible treatment option for patients with primary biliary cirrhosis An open-label study of rituximab, a monoclonal antibody for human CD20, was shown to be safe in patients with primary biliary cirrhosis who had an... Coffee consumption reduces fibrosis risk in those with fatty liver disease Caffeine consumption has long been associated with decreased risk of liver disease and reduced fibrosis in patients with chronic liver disease. Now, newly published research... Rearranging the cell's skeleton Cell biologists at Johns Hopkins have identified key steps in how certain molecules alter a cell's skeletal shape and drive the cell's movement. New ACS video celebrates the science behind one of Super Bowl Sunday's favorite foods With pizza, nachos and other cheesy dishes on the menu for an estimated 60 percent of Super Bowl Sunday football fans, the American Chemical Society,... EARTH: Dangerous dust What would you do if you found out that the roads you drive on could cause cancer? This is the reality that residents face in... Understanding how bacteria come back from the dead Salmonella remains a serious cause of food poisoning, in part due to its ability to thrive and quickly adapt to the different environments in which... The effect of occasional binge drinking on heart disease and mortality among moderate drinkers Most studies have found that binge drinking is associated with a loss of alcohol's protective effect against ischemic heart disease and most studies have found... UT biosolar breakthrough promises cheap, easy green electricity A professor of biochemistry, cellular and molecular biology, at the University of Tennessee, Knoxville, and a team of researchers have developed a system that taps... Potential new treatment identified for leishmaniasis Researchers at the University of Dundee have identified fexinidazole as a possible, much-needed, new treatment for the parasitic disease visceral leishmaniasis. Hand counts of votes may cause errors, says new Rice University study Hand counting of votes in postelection audit or recount procedures can result in error rates of up to two percent, according to a new study... Human immune cells react sensitively to 'stress' Scientists working with Professor Bernd Kaina of the Institute of Toxicology at the Medical Center of Johannes Gutenberg University Mainz have demonstrated for the first... Technology that translates content to the Internet protocol of the future A new protocol, IPv6, is being introduced across the Web. Researchers at Universidad Carlos III of Madrid who are participating in the Trilogy project have... Using plants to silence insect genes in a high-throughput manner Scientists at the Max Planck Institute for Chemical Ecology, Germany, are now using a procedure which brings forward ecological research on insects: They study gene... A silver bullet to beat cancer? The internet is awash with stories of how silver can be used to treat cancer. Now, lab tests have shown that it is as effective... Sanford-Burnham researchers find molecular switch that allows melanoma to resist therapy In a paper published Feb. 3 in Cell, researchers at Sanford-Burnham Medical Research Institute identified a molecular switch that controls the protein Activating Transcription Factor... Scripps research scientists demonstrate effective new 'biopsy in a blood test' to detect cancer Scientists from the Scripps Research Institute, Scripps Health, and collaborating cancer physicians have successfully demonstrated the effectiveness of an advanced blood test for detecting and... Study flags over-reliance on computer tests in return-to-plan decisions after concussion A new study by researchers at Indiana University-Purdue University Columbus and Pace University is critical of the widespread use of computerized neuropsychological tests in decisions... Heat and cold damage corals in their own ways, Scripps study shows Around the world coral reefs are facing threats brought by climate change and dramatic shifts in sea temperatures. While warming has been the primary focus... Gene regulator in brain's executive hub tracked across lifespan -- NIH study Scientists have tracked the activity, across the lifespan, of an environmentally responsive regulatory mechanism that turns genes on and off in the brain's executive hub.... 'Goldilocks' gene could determine best treatment for TB patients Tuberculosis patients may receive treatments in the future according to what version they have of a single 'Goldilocks' gene, says an international research team from... Sexually transmitted infections double in older population in 10 years Sexually active adults aged 45 and over are being encouraged to pay more thought to safe sex in line with recent figures showing that STIs... How to tell apart the forgetful from those at risk of Alzheimer's disease It can be difficult to distinguish between people with normal age-associated memory loss and those with amnestic mild cognitive impairment. However people with aMCI are... At International Stroke Conference, Cedars-Sinai neurology researchers present findings Stroke experts from Cedars-Sinai Medical Center will present research updates at the International Stroke Conference of the American Heart Association/American Stroke Association Feb. 1 through... Plant power: The ultimate way to 'go green'? Researchers are turning to plants and solar power in the search for new sources of renewable and sustainable energy that can support the transition from... NIH study uncovers probable mechanism underlying resveratrol activity National Institutes of Health researchers and their colleagues have identified how resveratrol, a naturally occurring chemical found in red wine and other plant products, may... Malaria kills nearly twice as many people than previously thought, but deaths declining rapidly Malaria is killing more people worldwide than previously thought -- 1.2 million -- but the number of deaths has fallen rapidly as efforts to combat... Intermittent exercise improves blood glucose control for diabetics Intermittent exercise with and without low oxygen concentrations can improve insulin sensitivity in type two diabetics, however exercise while under hypoxic conditions provides greater improvements...   [ Back ] News Feeds and Abstracts ISAC Updates and News Cytometry Part A Abstracts Cytometry Part B Abstracts Plant Flow Cytometry Blog Daily Dongle Feed Maekerlab Abstract Digest FlowCytometryNews.net EurekAlert! 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