MyCyte

Another arrow has been added to the flow cytometry quiver, as developments in protein translocation detection have combined electroporation with flow cytometry. Chang Lu, and a team at Purdue, have successfully monitored protein translocation for individual cells at a rate of 100-200 cells per second. Lu's study involved monitoring translocation of EGFP-tagged tyrosine kinase, Syk, to the plasma membrane in B cells.  Lu's work will continue, with the goal being the ability to monitor protein translocation for individual cells at standard flow cytometry rates.

Click here for Lu's abstract on this work.

For the ScienceDaily.com article on Lu's work click here .