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Microscopy images must be acquired at the optimal focal plane for the objects of interest in a scene. Although manual focusing is a standard task...
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Proper illumination is essential for light microscopy. Whereas in early years incandescent light was the only illumination, today, more and more specialized light sources, such...
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The Flow Cytometry Standard (FCS) format was developed back in 1984. Since then, FCS became the standard file format supported by all flow cytometry software...
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The development of polychromatic cytometry has contributed to significant progress in the field of human immunology. Although numerous functional studies of rare cell populations have...
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Acute promyelocytic leukemia (APL) is a hematological emergency in which a rapid diagnosis is essential for early administration of appropriate therapy, including all‐trans retinoic acid...
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In cerebrospinal fluid (CSF) analysis, hematology analyzers (HAs) Sysmex® XT‐4000i and XE‐5000, equipped with flow cytometry (FCM), were used to count cells and differentiate leukocytes...
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Currently, there is no standardized panel for immunophenotyping myeloid cells in mouse spleen using flow cytometry. Markers such as CD11b, CD11c, F4/80, Gr‐1, Ly6C, and...
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Cardiovascular disease (CVD) is now the largest killer in western society, and the importance of interactions between vascular endothelium and circulating blood components in disease...
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Amyloid beta (Aβ) oligomers and phosphorylated tau (p‐tau) aggregates are increasingly identified as potential toxic intermediates in Alzheimer's disease (AD). In cortical AD synapses, p‐tau...
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Neutrophils expel extracellular traps (NETs) to entrap and exterminate the invaded micro‐organisms. Acute/chronic inflammatory disorders are often observed with aberrantly enhanced NETs formation and high...
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Microbial adhesion is a field of recognized relevance and, as such, an impressive array of tools has been developed to understand its molecular mechanisms and...
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The inherent surface charges and small diameters that confer colloidal stability to gold particle conjugates (immunogold) are detrimental to rapid cell surface labeling and distinct...
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On the cover: Zebrafish vasculature portrayed in a whole new light on the cover of this issue. The source work by Zhang and coworkers in...
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Fluorescence resonance energy transfer (FRET) between fluorescent proteins (FPs) is a powerful method to visualize and quantify protein–protein interaction in living cells. Unfortunately, the emission...
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The combination of color‐coded microspheres as carriers and flow cytometry as a detection platform provides new opportunities for multiplexed measurement of biomolecules. Here, we developed...
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The measurement of γ‐H2AX foci induction in cells provides a sensitive and reliable method for the quantitation of DNA damage responses in a variety of...
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Presence of circulating tumor cells (CTC), as detected by the CellSearch® System, in patients with metastatic carcinomas is associated with poor survival prospects. CellTracks TDI,...
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Clusters of MHCI, ICAM‐1, CD44, CD59, IL‐2R, and IL‐15R molecules have been studied on the surface of CD4+ T‐cells from peripheral blood and lymph nodes...
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Erythroid biology research involving rhesus macaques has been applied to several topics including malaria, hemoglobinopathy and gene therapy research. However, analyses of the rhesus red...
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Investigations of rare cell types in peripheral blood samples, such as tumor, fetal, and endothelial cells, represent an emerging field with several potentially valuable medical...
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Adult zebrafish are being increasingly used as a model in cancer and stem cell research. Here we describe an integrated optical system that combines a...
Cytometry Part A Abstracts 
